Faculty of Oral & Dental Medicine
Research Facilities
A- Molecular Biology Laboratory
1) Biometra TProfessional Thermocycler 070-901 TProfessional 96
PCR Usage in medical field:
1) Diagnosing different diseases.
2) Detecting and characterizing pathogens in different medical fields as virology, mycology, parasitology, microbiology and dentistry
3) Efficacy of the drug therapy
2- SIGMA 3-30KS Refrigerated Centrifuge
The function of the device is to separate fluids, gas or liquid, based on the difference in density.
Three rotors are available with the centrifuge:
1) Rotor 12110-H; 12 of 2 ml tubes, max. speed 30,000 rpm, max load 12X5g
2) Rotor 1217/ 12 of 10-15 ml tubes, max. speed 13,500rpm, max. load 12X30g
3) Rotor 12155/ 5 of 85 ml tubes, max. speed 20,000rpm, max. load 4X250g
3) Polyacrylamide Gel Electrophoresis (PAGE) Sodium Dodecyl Sulfate-
Polyacrylamide Gel Electrophoresis (SDS-PAGE)
BIO-RAD Mini-PROTEAN Tetra Cell
It is a vertical mini gel electrophoresis system designed for fast, simple setup, facilitating rapid sample optimization, method development, and targeted protein analysis.
This technique is widely used in:
1) Separation of biological macromolecules, proteins and nucleic acids, from
mixture depending on their molecular weights and electrophoresis mobility
2) Detection of the presence of specific proteins related to specific diseases
3) Detection of the immune response
4) Enzyme-Linked Immunosorbent Assay (also called ELISA or EIA)
This is a test that is often used as a screening tool before using in depth diagnostic tools. Following are the most important equipment in ELISA technique:
– Plate washer which provides plate washing capability in a compact, efficient, and robust package. It can be easily programmed to wash a single strip, multiple strips or an entire plate.
– The BioTek ELx808 Absorbance Microplate Reader is a multi-channel absorbance microplate reader that can be used for research, development and diagnosis. The instrument functions as a spectrophotometer which measures intensity as a function of the color, or more specifically the wavelength of light.
This technique is widely used in:
1) Detection of the presence of specific proteins related to specific diseases
2) Detection of the immune response and measurement of antibodies against certain infectious diseases, antigens and proteins
B- Bacteriology Laboratory
This lab is used to identify:
1) bacterial organisms
2) antimicrobial resistance
3) the importance of bacteriology to bacterial diseases prevention and treatment
4) the effect of different agents on the bacterial growth
1) Binocular Microscope Model BM-180 (Boeco Laboratory)
Through its lens system, it facilitates the study of the invisible materials by the naked eye, including but not limited to, bacteria and tissue cells.
2) Incubator (Heraeus; Thermo Scientific)
Incubators provide a controlled, contaminant-free environment for safe, reliable work with bacteria, cell and tissue cultures by regulating conditions such as temperature, humidity, and CO2.
3) Class II Microbiological Safety Cabinet (Thermo Electron LED GmbH)
C- General Use Equipment:
Sartorius Basic Lite (BL) 210s Balance
It is an accurate, effective, and reliable weighing device used in quantitative analysis.
Allowable Ambient Operating Temperature: 5° C to 40 ° C (41° F to 104° F), 85% Relative humidity (RH) or less
Capacity(g): 210 gm.
Jenway 3330-Research pH Meter
PH meter is an electric device used to measure hydrogen-ion activity (acidity or alkalinity) in solution.
Series of Freezers (-20oC and -80oC)
Laboratory freezers provide an essential role in storage space for laboratory reagents and samples. All the freezers are equipped with adjustable shelves designed to accommodate multiple types of sample containers.
Vortex Mixer
(Fischer Scientific)
It is a simple device used commonly in laboratories to mix small volumes of liquid. It consists of an electric motor with the drive shaft oriented vertically and attached to a cupped rubber piece mounted slightly off-center.
Hot Plates and Stirrer
(JENWAY)
Hot plates used to heat glassware or its contents as a part of laboratory proceedure. Some hot plates contain a magnetic stirrer, allowing the heated liquid to be stirred using a magnetic bar.
D- Histopathology Lab:
The lab is used for:
A) processing soft tissue specimens for research and diagnostic purposes.
B) staining the processed and sectioned specimens, either with H. & E. or special stains to help in confirming the diagnosis of critical cases.
C) decalcification of hard tissue specimens and subsequent staining procedures.
I. Diagnostic procedures:
A) Performing microscopic examination of the prepared slides to reach final diagnosis of pathological lesions.
B) Writing histopathological reports for the examined slides.
C) Performing histopathological imaging to help in case studying, documentation and for educational and research purposes.
D) Using stereomicroscope for examining gross specimens (teeth, discs, casts…) and for analyzing data using the image j software.
This software can be used for calculating area, area percentage and linear measurements. It can also be used for performing cell count that is useful in research projects.
II. Immunocytochemistry
A specialized technique of histopathological examination that helps in the identification of specific targets (antigens) within cells and tissues. This involves the use of antibodies that are directed towards the specific targets of interest. The resulting antigen/antibody reaction is then visualized in the form of a different stain that is seen by microscopy.